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3gp Artis Bugil Ganti Baju Kamar Ganti Sarah Azhari,femmy,shanty Free Download->->->-> select null,null,null 3gp skodeng cewek abg dibius .Evaluation of a novel, in-hospital perfusion bioreactor system for tissue engineering.
Tissue engineering is a promising approach to treat and repair injured or diseased tissues, including heart, muscle and bone. Many in vitro bioreactor systems have been developed to examine the ability of tissues to be shaped and controlled in vitro. Our group has designed a novel in-hospital perfusion bioreactor to simulate an in vivo environment for tissue regeneration. The bioreactor was designed to be portable and could be easily integrated into a hospital setting. The perfusion bioreactor was first evaluated in-vivo for the formation of bone tissue. A tube-shaped, hydroxyapatite/poly(lactic-co-glycolic acid) scaffold was implanted within the femur of rabbit bone defects. The in-vivo studies demonstrated that the perfusion bioreactor, in-vivo simulated in-vitro conditions in a bioreactor system. A significant difference in the amount of new bone formation was observed between the bioreactor and a static bioreactor in-vivo (21.8+/-7.7% and 5.0+/-2.6% respectively). Our results demonstrate that an in-hospital perfusion bioreactor, in-vivo simulated in-vitro conditions in a bioreactor, will be an excellent model for tissue regeneration in-vitro, and may provide an alternative to the static bioreactor in-vivo.Background
==========

Fibrinogen is the main component of blood clots and has a well-known and established coagulant activity. Recently, we have found that fibrinogen has a direct binding to endothelial cells (EC) and induces migration and invasion of EC. The aim of this study was to examine whether fibrinogen could modulate invasion of human pancreatic cancer cell lines through human EC monolayer and examine the effect of anti-fibrinogen treatment on cell migration and invasion.

Methods
=======

Human pancreatic cancer cell lines Panc-1 and AsPC-1 were studied. Cell invasion was performed through Matrigel-coated filters.

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